Bio-assessing of environmental pollution via monitoring of Metallothionein level using electrochemical detection

Stejskal, Karel; Křížková, Soňa; Adam, Vojtěch; Sures, Bernd; Trnková, Libuše; Zehnálek, Josef; Hubálek, Jaromír; Beklová, Miroslava; Hanuštiak, Pavel; Svobodová, Zdenka; Horna, Aleš; Kizek, René

dc.title	Bio-assessing of environmental pollution via monitoring of Metallothionein level using electrochemical detection	en
dc.contributor.author	Stejskal, Karel
dc.contributor.author	Křížková, Soňa
dc.contributor.author	Adam, Vojtěch
dc.contributor.author	Sures, Bernd
dc.contributor.author	Trnková, Libuše
dc.contributor.author	Zehnálek, Josef
dc.contributor.author	Hubálek, Jaromír
dc.contributor.author	Beklová, Miroslava
dc.contributor.author	Hanuštiak, Pavel
dc.contributor.author	Svobodová, Zdenka
dc.contributor.author	Horna, Aleš
dc.contributor.author	Kizek, René
dc.relation.ispartof	IEEE Sensors Journal
dc.identifier.issn	1530-437X Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2008-09/2008-10
utb.relation.volume	8
utb.relation.issue	9-10
dc.citation.spage	1578
dc.citation.epage	1585
dc.event.title	5th IEEE Sensors Conference
dc.event.location	Daegu
utb.event.state-en	Democratic People's Republic of Korea
utb.event.state-cs	Severní Korea
dc.event.sdate	2006-10-22
dc.event.edate	2006-10-25
dc.type	article
dc.type	conferenceObject
dc.language.iso	en
dc.publisher	The Institute of Electrical and Electronics Engineers (IEEE)	en
dc.identifier.doi	10.1109/JSEN.2008.928500
dc.relation.uri	http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=4609975
dc.subject	Amperometric detection	en
dc.subject	biomarker	en
dc.subject	electrochemical sensors	en
dc.subject	fish	en
dc.subject	flow injection analysis	en
dc.subject	heavy metal	en
dc.subject	metallothionein	en
dc.subject	poecilia reticulata	en
dc.subject	silver	en
dc.description.abstract	In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1001850
utb.identifier.obdid	43857023
utb.identifier.obdid	43857370
utb.identifier.scopus	2-s2.0-85008013885
utb.identifier.wok	000260384900014
utb.source	d-wok
dc.date.accessioned	2011-08-09T07:34:05Z
dc.date.available	2011-08-09T07:34:05Z
utb.contributor.internalauthor	Horna, Aleš
utb.fulltext.affiliation	Karel Stejskal, Sona Krizkova, Vojtech Adam, Bernd Sures, Libuse Trnkova, Josef Zehnalek, Jaromir Hubalek, Miroslava Beklova, Pavel Hanustiak, Zdenka Svobodova, Ales Horna, and Rene Kizek K. Stejskal is with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic and also with the Department of Biochemistry, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic. S. Krizkova, J. Zehnalek, and R. Kizek are with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic (e-mail: kizek@sci.muni.cz). V. Adam is with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic and also with the Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic. B. Sures is with the Universität Duisburg-Essen, Applied Zoology and Hydrobiology, D-45117 Essen, Germany. L. Trnkova is with the Department of Chemistry, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic. J. Hubalek is with the Department of Microelectronics, Faculty of Electrical Engineering and Communication, Technical University of Brno, CZ-602 00 Brno, Czech Republic. M. Beklova and P. Hanustiak are with the Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, CZ-612 42 Brno, Czech Republic. Z. Svobodova is with the Institute of Parasitology, University of Veterinary and Pharmaceutical Sciences, CZ-612 42 Brno, Czech Republic. A. Horna is with the Faculty of Technology, Tomas Bata University, CZ-762 72 Zlin, Czech Republic.
utb.fulltext.dates	Manuscript received March 31, 2007 revised June 30, 2007 and September 28, 2007 accepted January 6, 2008
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utb.fulltext.sponsorship	This work was supported under Grants GACR 526/07/0808, MSMT 6215712402, MSMT 2636/F4b, and MSMT INCHEMBIOL 0021622412.
utb.fulltext.projects	GACR 526/07/0808
utb.fulltext.projects	MSMT 6215712402
utb.fulltext.projects	MSMT 2636/F4b
utb.fulltext.projects	MSMT INCHEMBIOL 0021622412