Different source atelocollagen thin films: Preparation, process optimisation and its influence on the interaction with eukaryotic cells

López García, Jorge Andrés; Humpolíček, Petr; Lehocký, Marián; Junkar, Ita; Mozetič, Miran

dc.title	Different source atelocollagen thin films: Preparation, process optimisation and its influence on the interaction with eukaryotic cells	en
dc.contributor.author	López García, Jorge Andrés
dc.contributor.author	Humpolíček, Petr
dc.contributor.author	Lehocký, Marián
dc.contributor.author	Junkar, Ita
dc.contributor.author	Mozetič, Miran
dc.relation.ispartof	Materiali in Tehnologije
dc.identifier.issn	1580-2949 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2013
utb.relation.volume	47
utb.relation.issue	4
dc.citation.spage	473
dc.citation.epage	479
dc.type	article
dc.language.iso	en
dc.publisher	Institut za Kovinske Materiale in Tehnologije Ljubljana	sl
dc.relation.uri	http://mit.imt.si/Revija/mit134.html
dc.subject	Atelocollagen thin films	en
dc.subject	Eukaryotic cell response	en
dc.subject	Film optimisation	en
dc.subject	Film quality	en
dc.subject	Surface topography	en
dc.description.abstract	Collagen thin films were prepared via bovine atelocollagen matrices. The film casting was carried out by using different culture dishes, concentrations, equipment, drying processes and periods of time. In order to optimise the repeatability and reproducibility, microscopic analyses were utilised to explore the film quality and topographical patterning. In addition, the human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded onto the obtained specimens, and the cell proliferation was determined by using the MTT assay. These results indicated how the substrate, its concentration and processing conditions influence the cellular response. The attempted technique shows itself to be an excellent procedure for continuous collagen film preparation with optimal cell-proliferation rates, which may potentially be used in tissue engineering or wound-healing applications.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1003443
utb.identifier.obdid	43869926
utb.identifier.scopus	2-s2.0-84881091292
utb.identifier.wok	000323085200013
utb.source	j-scopus
dc.date.accessioned	2013-08-21T10:18:13Z
dc.date.available	2013-08-21T10:18:13Z
dc.rights.uri	http://mit.imt.si/
dc.rights.access	openAccess
utb.contributor.internalauthor	López García, Jorge Andrés
utb.contributor.internalauthor	Humpolíček, Petr
utb.contributor.internalauthor	Lehocký, Marián
utb.fulltext.affiliation	Jorge López García1, Petr Humpolíček1, Marián Lehocký1, Ita Junkar2, Miran Mozetič2 1 Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic 2 Department of Surface Engineering, Plasma Laboratory, Jo`ef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia lehocky@post.cz
utb.fulltext.dates	Prejem rokopisa – received: 2012-11-16; sprejem za objavo – accepted for publication: 2013-01-09
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utb.fulltext.sponsorship	The authors would like to express their gratitude to the Ministry of Education, Youth and Sport of the Czech Republic (CZ.1.05/2.1.00/03.0111). The Slovenia Ministry of Higher Education, Science, and Technology (Program P2-0082-2) and Ad Futura L7-4009 are also gratefully acknowledged for the financial support of this research.