Polyphenolic extracts of edible flowers incorporated onto atelocollagen matrices and their effect on cell viability

López García, Jorge Andrés; Kuceková, Zdenka; Humpolíček, Petr; Mlček, Jiří; Sáha, Petr

dc.title	Polyphenolic extracts of edible flowers incorporated onto atelocollagen matrices and their effect on cell viability	en
dc.contributor.author	López García, Jorge Andrés
dc.contributor.author	Kuceková, Zdenka
dc.contributor.author	Humpolíček, Petr
dc.contributor.author	Mlček, Jiří
dc.contributor.author	Sáha, Petr
dc.relation.ispartof	Molecules
dc.identifier.issn	1420-3049 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2013
utb.relation.volume	18
utb.relation.issue	11
dc.citation.spage	13435
dc.citation.epage	13445
dc.type	article
dc.language.iso	en
dc.publisher	MDPI AG	en
dc.identifier.doi	10.3390/molecules181113435
dc.relation.uri	http://www.mdpi.com/1420-3049/18/11/13435
dc.subject	Atelocollagen thin films	en
dc.subject	Cell viability	en
dc.subject	Edible flowers	en
dc.subject	Polyphenolic extracts	en
dc.subject	Tissue engineering	en
dc.description.abstract	The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae), introduced Sage (Salvia pratensis, Lamiaceae), European elderberry (Sambucus nigra, Caprifoliaceae) and common dandelion (Taraxacum officinale, Asteraceae) were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen. © 2013 by the authors.	en
utb.faculty	University Institute
dc.identifier.uri	http://hdl.handle.net/10563/1003574
utb.identifier.obdid	43870233
utb.identifier.scopus	2-s2.0-84888587167
utb.identifier.wok	000330311500023
utb.identifier.coden	MOLEF
utb.source	j-scopus
dc.date.accessioned	2013-12-18T16:07:41Z
dc.date.available	2013-12-18T16:07:41Z
dc.description.sponsorship	European Regional Development Fund (ERDF); project Centre of Polymer Systems [CZ.1.05/2.1.00/03.0111]; Advanced Theoretical and Experimental Studies of Polymer Systems [CZ.1.07/2.3.00/20.0104]; Czech Science Foundation [13-08944S]; TBU in Zlin [IGA/FT/2013/019]
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access	openAccess
utb.contributor.internalauthor	López García, Jorge Andrés
utb.contributor.internalauthor	Kuceková, Zdenka
utb.contributor.internalauthor	Humpolíček, Petr
utb.contributor.internalauthor	Mlček, Jiří
utb.contributor.internalauthor	Sáha, Petr
utb.fulltext.affiliation	Jorge López-García 1, Zdenka Kuceková 1,2, Petr Humpolíček 1,2,, Jiři Mlček 3 and Petr Sáha 1 1 Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G.Masaryka-5555, Zlín 76001, Czech Republic; E-Mails: vextropk@gmail.com (J.L.-G.); kucekova@ft.utb.cz (Z.K.); saha@utb.cz (P.S.) 2 Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. sq. 275, Zlin 76272, Czech Republic 3 Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlin, nám. T.G.Masaryka-5555, Zlin 76272, Czech Republic; E-Mail: mlcek@ft.utb.cz Author to whom any correspondence should be addressed; E-Mail: humpolicek@ft.utb.cz; Tel.: +420-73-479-2298; Fax: +420-57-603-1444.
utb.fulltext.dates	Received: 30 August 2013; in revised form: 3 October 2013 / Accepted: 23 October 2013 / Published: 30 October 2013
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utb.fulltext.sponsorship	The authors would like to express their gratitude to the Operational Program Research and Development for Innovations co-funded by the European Regional Development Fund (ERDF) and national budget of Czech Republic, within the framework of project Centre of Polymer Systems (reg. number: CZ.1.05/2.1.00/03.0111), the Advanced Theoretical and Experimental Studies of Polymer Systems (reg. number: CZ.1.07/2.3.00/20.0104) and the Czech Science Foundation (13-08944S). Zdenka Kuceková would also like to thank the internal grant of TBU in Zlin No. IGA/FT/2013/019 funded for her financial support.
utb.fulltext.projects	CZ.1.05/2.1.00/03.0111
utb.fulltext.projects	CZ.1.07/2.3.00/20.0104
utb.fulltext.projects	13-08944S
utb.fulltext.projects	IGA/FT/2013/019