Enhancement of 5-aminolevulinic acid phototoxicity by encapsulation in polysaccharides based nanocomplexes for photodynamic therapy application

Di Martino, Antonio; Pavelková, Alena; Postnikov, Pavel S.; Sedlařík, Vladimír

dc.title	Enhancement of 5-aminolevulinic acid phototoxicity by encapsulation in polysaccharides based nanocomplexes for photodynamic therapy application	en
dc.contributor.author	Di Martino, Antonio
dc.contributor.author	Pavelková, Alena
dc.contributor.author	Postnikov, Pavel S.
dc.contributor.author	Sedlařík, Vladimír
dc.relation.ispartof	Journal of Photochemistry and Photobiology B: Biology
dc.identifier.issn	1011-1344 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2017
utb.relation.volume	175
dc.citation.spage	226
dc.citation.epage	234
dc.type	article
dc.language.iso	en
dc.publisher	Elsevier
dc.identifier.doi	10.1016/j.jphotobiol.2017.08.010
dc.relation.uri	https://www.sciencedirect.com/science/article/pii/S1011134417305328
dc.subject	5-aminolevulinic acid	en
dc.subject	controlled delivery chitosan	en
dc.subject	nanoparticles	en
dc.subject	photodynamic therapy	en
dc.subject	polysaccharides	en
dc.description.abstract	Polysaccharides based nanocomplexes have been developed for encapsulation, controlled delivery and to enhance the phototoxicity of the photosensitizer 5-aminolevulinic acid for application in photodynamic therapy. The nanocomplexes were prepared by coacervation in a solvent free environment using chitosan as polycation while alginic and polygalacturonic acid as polyanions. The complexes showed average dimension in the range 90–120 nm, good stability in simulated physiological media and high drug encapsulation efficiency, up to 800 μg per mg of carrier. Release studies demonstrate the possibility to tune the overall release rate and the intensity of the initial burst by changing the external pH. Cytotoxicity and photocytotoxicity tests confirmed the not toxicity of the used polysaccharides. Cell viability results confirmed the improvement of 5-aminolevulinic acid phototoxicity when loaded into the carrier compared to the free form. No effect of the irradiation on the nanocomplexes structure and on the release kinetics of the drug was observed. The results demonstrate that the prepared formulations have suitable properties for future application in photodynamic therapy and to ameliorate the therapeutic efficacy and overcome the side-effects related to the use of the photosensitizer 5-aminolevulinic acid. © 2017	en
utb.faculty	University Institute
dc.identifier.uri	http://hdl.handle.net/10563/1007497
utb.identifier.obdid	43877162
utb.identifier.scopus	2-s2.0-85029289691
utb.identifier.wok	000413177200028
utb.identifier.pubmed	28915492
utb.identifier.coden	JPPBE
utb.source	j-scopus
dc.date.accessioned	2017-10-16T14:43:39Z
dc.date.available	2017-10-16T14:43:39Z
dc.description.sponsorship	15-08287Y, GACR, Grantová Agentura České Republiky; CZ.1.05/2.1.00/19.0409, MŠMT, Ministerstvo Školství, Mládeže a Tělovýchovy; LO1504, MŠMT, Ministerstvo Školství, Mládeže a Tělovýchovy
dc.description.sponsorship	Czech Science Foundation [15-08287Y]; Ministry of Education, Youth and Sports of the Czech Republic [L01504, CZ.1.05/2.1.00/19.0409]; Tomsk Polytechnic University [VIU-316/2017]
utb.ou	Centre of Polymer Systems
utb.contributor.internalauthor	Di Martino, Antonio
utb.contributor.internalauthor	Pavelková, Alena
utb.contributor.internalauthor	Sedlařík, Vladimír
utb.fulltext.affiliation	Antonio Di Martino a,b,⁎ , Alena Pavelkova a , Pavel S. Postnikov b , Vladimir Sedlarik a a Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic b Tomsk Polytechnic University, Lenin Av. 30, 634050 Tomsk, Russian Federation * Corresponding author at: Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic. E-mail address: dimartino@utb.cz (A. Di Martino).
utb.fulltext.dates	Received 20 April 2017 Accepted 6 August 2017 Available online 08 August 2017
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utb.fulltext.sponsorship	This work was funded by the Czech Science Foundation (grant no. 15-08287Y), Ministry of Education, Youth and Sports of the Czech Republic (grant no. LO1504 and CZ.1.05/2.1.00/19.0409) and Tomsk Polytechnic University (project VIU-316/2017).
utb.scopus.affiliation	Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, Zlin, Czech Republic; Tomsk Polytechnic University, Lenin Av. 30, Tomsk, Russian Federation