Characterization of Escherichia Coli strains isolated from raw vegetables

Janalíková, Magda; Pleva, Pavel; Pavlíčková, Silvie; Lecomte, Martin; Godillon, Tanguy; Holko, Ivan

dc.title	Characterization of Escherichia Coli strains isolated from raw vegetables	en
dc.contributor.author	Janalíková, Magda
dc.contributor.author	Pleva, Pavel
dc.contributor.author	Pavlíčková, Silvie
dc.contributor.author	Lecomte, Martin
dc.contributor.author	Godillon, Tanguy
dc.contributor.author	Holko, Ivan
dc.relation.ispartof	Potravinarstvo Slovak Journal of Food Sciences
dc.identifier.issn	1338-0230 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2018
utb.relation.volume	12
utb.relation.issue	1
dc.citation.spage	304
dc.citation.epage	312
dc.type	article
dc.language.iso	en
dc.publisher	HACCP Consulting
dc.identifier.doi	10.5219/897
dc.relation.uri	http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/897
dc.subject	Escherichia coli	en
dc.subject	Resistance	en
dc.subject	Safety	en
dc.subject	Vegetables	en
dc.subject	Virulence	en
dc.description.abstract	Vegetables are an important part of the human diet. Sometimes, contamination by pathogenic Escherichia coli can be underestimated; moreover there is a risk of antibiotic resistance spreading via the food chain. The purpose of this study was to examine the prevalence of Escherichia coli in fresh vegetables sold in retail market in the Czech Republic and to evaluate the risk to human health. Antibiotic resistance against 12 antibiotics, the presence of 12 virulence and 15 resistance genes were determined among 15 isolated strains. Most of tested strains belonged to B1 phylogenetic group, less frequently represented was B2 and D phylogroup. These results indicate that most strains are probably of human origin. All E. coli strains were resistant to at least one of twelve tested antibiotics. A multidrug resistance was observed in four strains. In this study, the presence of virulence factors Einv and papC and also genes encoding toxins (CNF1, CNF2) was detected. Nevertheless, none strain can be considered as STEC or EHEC. The widespread appearance of a growing trend associated with the prevalence of antibiotic resistance among enterobacterial isolates is undeniable and the possibility of transfer to humans cannot be ignored. Nevertheless, these results indicate that raw vegetables sold in the retail market can constitute a potential health risk for consumers. © 2018 Potravinarstvo Slovak Journal of Food Sciences.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1007829
utb.identifier.obdid	43878927
utb.identifier.scopus	2-s2.0-85044616745
utb.source	j-scopus
dc.date.accessioned	2018-04-23T15:01:47Z
dc.date.available	2018-04-23T15:01:47Z
dc.rights	Attribution 3.0 International
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.rights.access	openAccess
utb.contributor.internalauthor	Janalíková, Magda
utb.contributor.internalauthor	Pleva, Pavel
utb.contributor.internalauthor	Pavlíčková, Silvie
utb.fulltext.affiliation	Magda Janalíková, Pavel Pleva, Silvie Pavlíčková, Martin Lecomte, Tanguy Godillon, Ivan Holko Contact addresses: Magda Janalíková, Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic, corresponding author, Tel.: 00420 576 031 020; E-mail: mdolezalova@ft.utb.cz Pavel Pleva, Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic, Tel.: 00420 576 031 209; E-mail: ppleva@utb.cz Silvie Pavlíčková, Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic; E-mail: silvie.pavlickova@seznam.cz Martin Lecomte, Institute Universitaire de Technologie, Université d'Angers, 40, rue de Rennes - BP 73532, 49035 - Angers cedex 01, France; E-mail: martin.lcmt@gmail.com Tanguy Godillon, Institute Universitaire de Technologie, Université d'Angers, 40, rue de Rennes - BP 73532, 49035 - Angers cedex 01, France; E-mail: tanguygodillon79@live.fr Ivan Holko, Vetservis s.r.o., Kalvária 715/3, 949 01 Nitra, Slovak Republic; Tel.: 00421 376 519 742, E-mail: ivan.holko@gmail.com
utb.fulltext.dates	Received: 10 February 2018 Accepted: 16 March 2018 Available online: 24 April 2018
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utb.scopus.affiliation	Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, Zlín, Czech Republic; Institute Universitaire de Technologie, Université d'Angers, 40, rue de Rennes - BP 73532, Angers cedex 01, France; Vetservis s.r.o., Kalvária 715/3, Nitra, Slovakia
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