Enhancement of the antioxidant activity and stability of β-carotene using amphiphilic chitosan/nucleic acid polyplexes

Di Martino, Antonio; Trusova, Marina E.; Postnikov, Pavel S.; Sedlařík, Vladimír

dc.title	Enhancement of the antioxidant activity and stability of β-carotene using amphiphilic chitosan/nucleic acid polyplexes	en
dc.contributor.author	Di Martino, Antonio
dc.contributor.author	Trusova, Marina E.
dc.contributor.author	Postnikov, Pavel S.
dc.contributor.author	Sedlařík, Vladimír
dc.relation.ispartof	International Journal of Biological Macromolecules
dc.identifier.issn	0141-8130 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2018
utb.relation.volume	117
dc.citation.spage	773
dc.citation.epage	780
dc.type	article
dc.language.iso	en
dc.publisher	Elsevier
dc.identifier.doi	10.1016/j.ijbiomac.2018.06.006
dc.relation.uri	https://www.sciencedirect.com/science/article/pii/S0141813018312789
dc.subject	Polyplexes	en
dc.subject	Antioxidants	en
dc.subject	beta-carotene	en
dc.subject	Chitosan	en
dc.subject	Polylactic acid	en
dc.subject	Deoxyribonucleic acid	en
dc.description.abstract	β-carotene is a natural compound with significant antioxidant activity. However, its poor solubility in water and low stability reduce its potential application. Innovative polyplexes based on the combination of amphiphilic chitosan assembled with DNA have been developed using a solvent-free, simple and low-cost method with the aim to load, retain and enhance the antioxidant capability of β-carotene. The polyplexes, with dimension about 100 nm, and excellent stability, were able to hold up to 400 μg of β-carotene per mg of the carrier, with minimal loss till two weeks. The antioxidant activity was significantly enhanced after loading, as demonstrated using two well known methods. Cytotoxicity assay confirmed the not toxicity of the system. The results suggest the polyplexes as an excellent candidate to develop formulation able to preserve and enhance the peculiarities of compounds which are used mainly in food, cosmetic and pharmaceutic but with still some limitations. © 2018	en
utb.faculty	University Institute
dc.identifier.uri	http://hdl.handle.net/10563/1007951
utb.identifier.obdid	43878241
utb.identifier.scopus	2-s2.0-85048001660
utb.identifier.wok	000442057700089
utb.identifier.pubmed	29874555
utb.identifier.coden	IJBMD
utb.source	j-scopus
dc.date.accessioned	2018-07-27T08:47:35Z
dc.date.available	2018-07-27T08:47:35Z
dc.description.sponsorship	VIU-RSCABS-89/2018, TPU, Tomsk Polytechnic University; NPU I LO1504
dc.description.sponsorship	Ministry of Education, Youth and Sports of the Czech Republic [NPU I LO1504]; Tomsk Polytechnic University [VIU-RSCABS-89/2018]
utb.ou	Centre of Polymer Systems
utb.contributor.internalauthor	Di Martino, Antonio
utb.contributor.internalauthor	Sedlařík, Vladimír
utb.fulltext.affiliation	Antonio Di Martino a,b, * , Marina E. Trusova b , Pavel S. Postnikov b , Vladimir Sedlarik a a Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr.Tomas Bati, 5678, 76001 Zlin, Czech Republic b Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Lenin Av. 30, 634050 Tomsk, Russian Federation * Corresponding author. E-mail address: dimartino@utb.cz (A. Di Martino).
utb.fulltext.dates	Received 16 March 2018 Received in revised form 1 June 2018 Accepted 2 June 2018 Available online 03 June 2018
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utb.fulltext.sponsorship	This work was funded by the Ministry of Education, Youth and Sports of the Czech Republic (grant no. NPU I LO1504) and Tomsk Polytechnic University (project VIU-RSCABS-89/2018).
utb.wos.affiliation	[Di Martino, Antonio; Sedlarik, Vladimir] Tomas Bata Univ Zlin, Univ Inst, Ctr Polymer Syst, Tr Tomas Bati 5678, Zlin 76001, Czech Republic; [Di Martino, Antonio; Trusova, Marina E.; Postnikov, Pavel S.] Tomsk Polytech Univ, Res Sch Chem & Appl Biomed Sci, Lenin Av 30, Tomsk 634050, Russia
utb.scopus.affiliation	Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr.Tomas Bati, 5678, Zlin, Czech Republic; Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Lenin Av. 30, Tomsk, Russian Federation
utb.fulltext.projects	VIU-RSCABS-89/2018