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Versatile digital polymerase chain reaction chip design, fabrication, and image processing

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dc.title Versatile digital polymerase chain reaction chip design, fabrication, and image processing en
dc.contributor.author Li, Huanan
dc.contributor.author Zhang, Haoqing
dc.contributor.author Xu, Ying
dc.contributor.author Turečková, Alžběta
dc.contributor.author Zahradník, Pavel
dc.contributor.author Chang, Honglong
dc.contributor.author Neužil, Pavel
dc.relation.ispartof Sensors and Actuators, B: Chemical
dc.identifier.issn 0925-4005 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2019
utb.relation.volume 283
dc.citation.spage 677
dc.citation.epage 684
dc.type article
dc.language.iso en
dc.publisher Elsevier
dc.identifier.doi 10.1016/j.snb.2018.12.072
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0925400518321919
dc.subject Digital PCR en
dc.subject Silicon chip en
dc.subject Fluorescence image analysis en
dc.subject DNA counting en
dc.subject Microfluidic chip en
dc.subject Array en
dc.description.abstract We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications. © 2018 Elsevier B.V. en
utb.faculty Faculty of Applied Informatics
dc.identifier.uri http://hdl.handle.net/10563/1008388
utb.identifier.obdid 43878996
utb.identifier.scopus 2-s2.0-85058703025
utb.identifier.wok 000455854000082
utb.identifier.coden SABCE
utb.source j-scopus
dc.date.accessioned 2019-01-31T08:58:58Z
dc.date.available 2019-01-31T08:58:58Z
dc.description.sponsorship "Foreign Experts Program" of PR China [W099109]; Grant Agency of the Czech Republic [GA16-11140S]
utb.contributor.internalauthor Turečková, Alžběta
utb.fulltext.affiliation Huanan Li a, Haoqing Zhang a, Ying Xu a, Alzbeta Tureckova a,b, Pavel Zahradník a,c, Honglong Chang a⁎, Pavel Neuzil a,d⁎ a Northwestern Polytechnical University, Department of Microsystems, Faculty of Mechanical Engineering, 127 West Youyi Road, Xi’an, 710072 Shaanxi, PR China b Tomas Bata University in Zlin, Faculty of Applied Informatics, Nam T.G. Masaryka 5555, 760 01 Zlin, Czech Republic c Czech Technical University in Prague, Faculty of Electrical Engineering, Department of Telecommunications Engineering, Technicka 1902/2, 166 27, Czech Republic d Brno University of Technology, Faculty of Electrical Engineering and Communication, SIX Centre, Department of Microelectronics, Technická 3058/10, 61600 Brno, Czech Republic ⁎ Corresponding authors at: Northwestern Polytechnical University, Department of Microsystems, Faculty of Mechanical Engineering, 127 West Youyi Road, Xi’an, 710072 Shaanxi, PR China.
utb.fulltext.dates Received 13 August 2018 Received in revised form 24 November 2018 Accepted 13 December 2018 Available online 14 December 2018
utb.scopus.affiliation Northwestern Polytechnical University, Department of Microsystems, Faculty of Mechanical Engineering, 127 West Youyi Road, Xi'an, Shaanxi 710072, China; Tomas Bata University in Zlin, Faculty of Applied Informatics, Nam T.G. Masaryka 5555, Zlin, 760 01, Czech Republic; Czech Technical University in Prague, Faculty of Electrical Engineering, Department of Telecommunications Engineering, Technicka 1902/2166 27, Czech Republic; Brno University of Technology, Faculty of Electrical Engineering and Communication, SIX Centre, Department of Microelectronics, Technická 3058/10, Brno, 61600, Czech Republic
utb.fulltext.faculty Faculty of Applied Informatics
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