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Title: | Improved stability and efficacy of chitosan/pDNA complexes for gene delivery | ||||||||||
Author: | Cifani, Noemi; Chronopoulou, Laura; Pompili, Barbara; Di Martino, Antonio; Bordi, Federico; Sennato, Simona; Di Domenico, Enea Gino; Palocci, Cleofe; Ascenzioni, Fiorentina | ||||||||||
Document type: | Peer-reviewed article (English) | ||||||||||
Source document: | Biotechnology Letters. 2015, vol. 37, issue 3, p. 557-565 | ||||||||||
ISSN: | 0141-5492 (Sherpa/RoMEO, JCR) | ||||||||||
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DOI: | https://doi.org/10.1007/s10529-014-1727-7 | ||||||||||
Abstract: | Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy. | ||||||||||
Full text: | https://link.springer.com/article/10.1007/s10529-014-1727-7 | ||||||||||
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