Preparation of collagen-elastin hydrolysates from cattle tendons: Preparation conditions

Abstract

This study deals with preparing collagen-elastin hydrolysates from cattle tendons through a 3-stage process. In the 1st stage, tendons are processed in an acidic, neutral or alkaline environment which produces their swelling. In the 2nd stage, a suitable proteolytic enzyme acts in neutral environment which leads to disrupt bonding interactions of collagen and elastin. In the 3rd stage, hydrolysate is extracted at elevated temperature from material so prepared. Variables under study were the environment in 1st stage processing (pH 4-11), duration (2-14 min) and temperature (60-100°C) in 3rd stage processing on process efficiency (hydrolysate yield). Tests are planned and evaluated by the method of statistic factor experiments. Maximum process efficiency (76 %) was found with tendons treated in an alkaline environment. Prepared hydrolysates of collagen-elastin type, light-yellow powder, may be employed in cosmetics or the foodstuffs industry.