Polypyrrole nanotubes decorated with gold particles applied for construction of enzymatic bioanodes and biocathodes

Abstract

The novel composites of gold nanoparticles and polypyrrole nanotubes (Au-x@PNT) were prepared and used as a platform for fabrication of bioelectrode interfaces. Changing the conditions of composite preparation caused variations in a gold architecture, electrical conductivity and a biocompatibility. These features could be easily adjusted by setting up a proper fabrication protocol. The Au-x@PNT-chitosan matrix was utilized for fabrication of bioelectrode interfaces with physisorbed oxidoreductases. Biocatalytic activity of such physisorbed fructose dehydrogenase (FDH), laccase (Lac) and bilirubin oxidase (BOD) was investigated with biocatalytic current density up to j = 2.45 mA cm(-2) obtained for a bioanode based on direct electron transfer of FDH. Performance of biocathodes with immobilized Lac and BOD showed current density up to 232 mu A cm(-2) in presence of a redox mediator.